Guanine nucleotide-binding protein G sub(o)-induced coupling of neuropeptide Y receptors to Ca super(2+) channels in sensory neurons

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 85; no. 10; pp. 3633 - 3637
• Main Authors: Ewald, DA, Sternweis, P C, Miller, R J
• Format: Journal Article
• Language: English
• Published: 01-01-1988
• ISSN: 0027-8424

Neuropeptide Y (NPY) inhibited the Ca super(2+) current (I sub(Ca)) in rat dorsal root ganglion neurons in vitro. When a combination of GTP and the purified alpha -subunit of the guanine nucleotide-binding protein G sub(o) was perfused into PTX-treated cells, the inhibitory effects of NPY on the I sub(Ca) reappeared in a time-dependent fashion. GTP or alpha -subunit perfused separately was relatively ineffective. The results indicate that G sub(o), the major PTX substrate in the central nervous system, may normally mediate the inhibitory effects of NPY receptors on dorsal root ganglion Ca super(2+) channels.