Increased plasma membrane permeability to Ca super(2+) in anti-Ig-stimulated B lymphocytes is dependent on activation of phosphoinositide hydrolysis

• Published in: The Journal of immunology (1950) Vol. 140; no. 9; pp. 3150 - 3155
• Main Authors: Ransom, J T, Chen, M, Sandoval, V M, Pasternak, JA, Digiusto, D, Cambier, J C
• Format: Journal Article
• Language: English
• Published: 01-01-1988
• ISSN: 0022-1767

The biochemical basis of Ca super(2+) mobilization after anti-Ig binding to B cell Ag-R has been further characterized by flow cytometric analysis of indo-1-loaded B cells. The ability to distinguish intracellular Ca super(2+) release from extracellular Ca super(2+) influx by using an extracellular calcium depletion-repletion approach has allowed the authors to study the relationship between the mobilization of Ca super(2+) from these sources. Studies involving manipulation of the Ca super(2+) gradient across the plasma membrane indicate that a significant portion of the Ca super(2+) mobilization response is preserved even when the normal inwardly directed Ca super(2+) gradient is reversed.