Integrated Analysis of Global mRNA and Protein Expression Data in HEK293 Cells Overexpressing PRL-1: e72977

• Published in: PloS one Vol. 8; no. 9
• Main Authors: Dumaual, Carmen M, Steere, Boyd A, Walls, Chad D, Wang, Mu, Zhang, Zhong-Yin, Randall, Stephen K
• Format: Journal Article
• Language: English
• Published: 01-09-2013
• Subjects: Cell migration
• ISSN: 1932-6203
• DOI: 10.1371/journal.pone.0072977

Background The protein tyrosine phosphatase PRL-1 represents a putative oncogene with wide-ranging cellular effects. Overexpression of PRL-1 can promote cell proliferation, survival, migration, invasion, and metastasis, but the underlying mechanisms by which it influences these processes remain poorly understood. Methodology To increase our comprehension of PRL-1 mediated signaling events, we employed transcriptional profiling (DNA microarray) and proteomics (mass spectrometry) to perform a thorough characterization of the global molecular changes in gene expression that occur in response to stable PRL-1 overexpression in a relevant model system (HEK293). Principal Findings Overexpression of PRL-1 led to several significant changes in the mRNA and protein expression profiles of HEK293 cells. The differentially expressed gene set was highly enriched in genes involved in cytoskeletal remodeling, integrin-mediated cell-matrix adhesion, and RNA recognition and splicing. In particular, members of the Rho signaling pathway and molecules that converge on this pathway were heavily influenced by PRL-1 overexpression, supporting observations from previous studies that link PRL-1 to the Rho GTPase signaling network. In addition, several genes not previously associated with PRL-1 were found to be significantly altered by its expression. Most notable among these were Filamin A, RhoGDI alpha , SPARC, hnRNPH2, and PRDX2. Conclusions and Significance This systems-level approach sheds new light on the molecular networks underlying PRL-1 action and presents several novel directions for future, hypothesis-based studies.