Analysis of circulating lung cancer cells in the peripheral blood in patients with lung cancer by flow cytometry

• Published in: Zhongguo fei ai za zhi Vol. 4; no. 2; pp. 102 - 104
• Main Authors: Sha, H, Jiang, X, Gu, W, Bao, G, Feng, J, Dong, Q
• Format: Journal Article
• Language: Chinese
• Published: 20-04-2001
• ISSN: 1009-3419
• DOI: 10.3779/j.issn.1009-3419.2001.02.07

BACKGROUNDTo analyze circulating lung cancer cells in the peripheral blood in patients with lung cancer by flow cytometry (FCM).METHODSThe monocyte fraction in peripheral blood was isolated by Ficoll-Hypaque gradient centrifugation. The cells obtained were labeled with antibodies against CD45, cytokeratin (CK) and antigen (2F7/S5A). The CD45(-) CK(+) 2F7/S5A(+) cells were analyzed by FCM.RESULTSFifty cases out of 165 patients with lung cancer (30.30%) were found to have cancer cells in the peripheral blood. Positive rate in non-small cell lung cancer was 30.67%(45/150) and that in small cell lung cancer was 33.33%(5/15) respectively. Meanwhile, there was distinct correlation between detective rate of cancer cells in the peripheral blood and pathological stage(P<0.05).CONCLUSIONSExamination of lung cancer presented in the peripheral blood by FCM might be helpful for staging and finding metastatic potential of lung cancer.