Structure of the O-specific polysaccharide of the marine bacterium Arenibacter palladensis KMM 3961^sup T^ containing 2-acetamido-2-deoxy-L-galacturonic acid

• Published in: Biochemistry (Moscow) Vol. 77; no. 1; p. 87
• Main Authors: Tomshich, S V, Isakov, V V, Komandrova, N A, Shevchenko, L S
• Format: Journal Article
• Language: English
• Published: New York Springer Nature B.V 01-01-2012
• Subjects: Amino acids; Bacteria; Biodegradation; NMR; Nuclear magnetic resonance
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1134/S0006297912010105

The O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Arenibacter palladensis type strain KMM 3961^sup T^ and studied by chemical methods and ^sup 1^H and ^sup 13^C NMR spectroscopy including 2D COSY, TOCSY, ^sup 1^H,^sup 13^C HSQC, and HMBC experiments. The polysaccharide was shown to consist of tetrasaccharide repeating units containing two mannose residues (Man), one 2-acetamido-2-deoxy-D-galactose residue (D-GalNAc), and one 2-acetamido-2-deoxy-L-galacturonic acid residue (L-GalNAcA) and having the following structure: → 2 ) − α − D − Manp − ( 1 → 6 ) − α − D − Manp − ( 1 → 4 ) − α − L − GalpNAcA − ( 1 → 3 ) − β − D − GalpNAc − ( 1 → .[PUBLICATION ABSTRACT]