Solid-State ^sup 2^H NMR Shows Equivalence of Dehydration and Osmotic Pressures in Lipid Membrane Deformation

• Published in: Biophysical journal Vol. 100; no. 1; p. 98
• Main Authors: Mallikarjunaiah, KJ, Leftin, Avigdor, Kinnun, Jacob J, Justice, Matthew J, Rogozea, Adriana L, Petrache, Horia I, Brown, Michael F
• Format: Journal Article
• Language: English
• Published: New York Biophysical Society 05-01-2011
• Subjects: Deformities; Dehydration; Lipids; Membranes; NMR; Nuclear magnetic resonance; Osmosis; Proteins; Solid state physics; Spectrum analysis; Thermodynamics
• ISSN: 0006-3495; 1542-0086

Lipid bilayers represent a fascinating class of biomaterials whose properties are altered by changes in pressure or temperature. Functions of cellular membranes can be affected by nonspecific lipid-protein interactions that depend on bilayer material properties. Here we address the changes in lipid bilayer structure induced by external pressure. Solid-state ...H NMR spectroscopy of phospholipid bilayers under osmotic stress allows structural fluctuations and deformation of membranes to be investigated. We highlight the results from NMR experiments utilizing pressure-based force techniques that control membrane structure and tension. Our ...H NMR results using both dehydration pressure (low water activity) and osmotic pressure (poly(ethylene glycol) as osmolyte) show that the segmental order parameters (S...) of DMPC approach very large values of ...0.35 in the liquid-crystalline state. The two stresses are thermodynamically equivalent, because the change in chemical potential when transferring water from the interlamellar space to the bulk water phase corresponds to the induced pressure. This theoretical equivalence is experimentally revealed by considering the solid-state ...H NMR spectrometer as a virtual osmometer. Moreover, we extend this approach to include the correspondence between osmotic pressure and hydrostatic pressure. Our results establish the magnitude of the pressures that lead to significant bilayer deformation including changes in area per lipid and volumetric bilayer thickness. We find that appreciable bilayer structural changes occur with osmotic pressures in the range of 10...100 atm or lower. This research demonstrates the applicability of solid-state ...H NMR spectroscopy together with bilayer stress techniques for investigating the mechanism of pressure sensitivity of membrane proteins. (ProQuest: ... denotes formulae/symbols omitted.)