Oil-Degrading Microorganisms of the Genus Pseudomonas with Resistance to Additional Pollutants and Phytostimulating Activity as Promising Agents of Environmental Biotechnology

• Published in: Žurnal Sibirskogo federalʹnogo universiteta. Seriâ Biologiâ (Online) Vol. 17; no. 1; pp. 64 - 81
• Main Authors: Korshunova, Tatiana Yu, Kuzina, Elena V, Iskuzhina, Milyausha G, Mukhamatdyarova, Svetlana R, Sharipova, Yuliyana Yu
• Format: Journal Article
• Language: Russian
• Published: Krasnoyarsk Siberian Federal University 01-01-2024
• Subjects: 2,4-D; Acetic acid; Agricultural land; Agricultural pollution; Alcaligenes; Bacteria; Bioremediation; Biotechnology; Cleaning; Decomposers; Decomposition; Heavy metals; Herbicides; Metals; Microorganisms; Nickel; Nitrogen fixation; Nitrogenation; Oil; Oil pollution; Oils & fats; Oxidation; Phosphates; Plant bacterial diseases; Plants; Pollutants; Pseudomonas; Reagents; Seedlings; Soil; Soil contamination; Soil pollution; Soils; Stimulators; Strains (organisms); Zinc
• ISSN: 1997-1389; 2313-5530

Bioremediation of oil contaminated agricultural soils is greatly hindered by the presence of additional pollutants such as herbicides. They can inhibit the activity of introduced bacterial decomposers. The aim of the study is to screen for herbicide-resistant oil-degrading microorganisms and to evaluate their ability to stimulate growth and development of phytomeliorant plants. Isolated and identified strains of hydrocarbon-oxidizing pseudomonads were found resistant to herbicides based on various active ingredients (2,4-D, imazethapyr, mixtures of imazamox and quizalofop-P-ethyl), as well as to heavy metals such as lead, nickel and zinc. All bacteria exhibited nitrogen-fixing and phosphate-mobilizing activity and produced indolyl-3-acetic acid. They helped plants to overcome stress caused by the presence of pollutants. They contributed to an increase in the length of the roots and shoots of barley and clover in clean and oil-contaminated soil and had a positive effect on clover seedlings in the presence of a herbicide. The P. frederiksbergensis UOM 11 strain was found to have a significant ability to decompose oil; it also enhanced growth and development of plants. Therefore, it is recommended as the basis for microbial-plant complexes for cleaning and restoring soils. The other strains obtained in screening are also valuable from the perspective of environmental biotechnology: P. alcaligenes UOM 10 is an effective oil-destructor; the other microorganisms are prospective growth stimulators for phytomeliorant plants. The results obtained can be used in the development of bioremediation methods for oil-contaminated agricultural soils.