OUTER MEMBRANE PROTEINS OF NONTYPABLE HAEMOPHILUS INFLUENZAE AS POSSIBLE MARKERS OF PATHOGENICITY IN PATIENTS WITH CHRONIC BRONCHITIS
A distinct role for nontypable Haemophilus influenzae in the pathogenesis of disease has not been described. The frequent recovery of this organism from patients with chronic bronchitis led me to examine the possibility that individual strains of H. influenzae may be responsible for acute exacerbati...
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| Format: | Dissertation |
| Language: | English |
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ProQuest Dissertations & Theses
01-01-1986
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| Online Access: | Get full text |
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| Summary: | A distinct role for nontypable Haemophilus influenzae in the pathogenesis of disease has not been described. The frequent recovery of this organism from patients with chronic bronchitis led me to examine the possibility that individual strains of H. influenzae may be responsible for acute exacerbations of this disease. Ninety-seven isolates of nontypable H. influenzae were isolated from patients with chronic bronchitis. The isolates were biotyped and their outer membrane protein (OMP) patterns were determined by SDS-PAGE. Ten OMP subtypes were determined on the basis of two major OMP. Subtype 7 was the most common isolate (41%) while subtypes 1 and 8 were only found in patients with acute disease (P < 0.00005). Examination of sera from patients with and without chronic bronchitis by Western immunoblot showed that only patients with chronic bronchitis develop antibodies to the major OMP of H. influenzae. The immunoblot procedure was superior to SDS-PAGE in demonstrating OMP profiles. These data suggest that the major OMP may play a role in the virulence of nontypable H. influenzae and can serve as antigenic markers in patients with acute chronic bronchitis. |
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| ISBN: | 9798206868838 |