mRna Expression of Ezrin in Gingival Crevicular Fluid and Whole Blood of Gingivitis and Chronic Periodontitis Patients- A Polymerase Chain Reaction Study

• Main Author: Cecil, Anju
• Format: Dissertation
• Language: English
• Published: ProQuest Dissertations & Theses 01-01-2019
• Subjects: Antigens; Bacteria; Biomarkers; Breast cancer; Cancer therapies; Cell adhesion & migration; Cellular biology; Cystic fibrosis; Cytoskeleton; Dentistry; Endothelium; Glycoproteins; Infections; Lymphocytes; Metastasis; Oncology; Pathogens; Pharmaceutical sciences; Phosphorylation; Physiology; Plasma; Polymerase chain reaction; Public health; Signal transduction; Tumor necrosis factor-TNF; Tumors; Womens health
• ISBN: 9798380282482

BACKGROUND AND OBJECTIVE:A comparative analysis of protein expression of GCF obtained from healthy individuals and individuals with periodontal diseases would help to identify proteins involved in periodontal disease progression. Among the identified proteins, Moesin which is a disease associated protein that belong to the Ezrin- Radixin- Moesin (ERM) protein family was proved to play an important role in the recognition of oral bacteria and in the consequent development of inflammatory immune responses involved in periodontal disease development. The aim of the present study is to quantify and compare mRNA expression levels of Ezrin in GCF and whole blood of gingivitis and chronic periodontitis patients.METHODS:A total of 60 patients were selected for the study and were divided into three groups as follows: Group I (20 participants with healthy gingiva), Group II (20 participants with gingivitis) and Group III (20 participants with chronic periodontitis). Clinical parameters like Gingival index, periodontal index, probing pocket depth and clinical attachment level were assessed. GCF and blood samples were taken from these patients and assessed for the mRNA expression of Ezrin using real time polymerase chain reaction (RT-PCR)RESULTS:The expression and mean relative quantification of mRNA expression of Ezrin in GCF and blood was higher for periodontitis (18.32±8.398,19.34±9.487) when compared to that of gingivitis (5.34±3.609,5.48±4.428) and healthy individuals (2.33±0.643, 3.47±1.923) and they positively correlated with the clinical parameters.CONCLUSION:The increased expression of Ezrin can be considered as a good indicator to assess the inflammatory activity in periodontitis and gingivitis.