Identification of chromosomal-related abnormalities in Pfeiffer cell line using Fluorescence in situ hybridization
[...]nuclei quality and chromosomes spreads were examined by using microscope. [...]the hybridization is affected by many important factors that have a fundamental role in the hybridization process such as temperature, duration of the hybridization time, the activity of the post-hybridization washes...
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| Published in: | Journal of pharmaceutical sciences and research Vol. 11; no. 3; pp. 774 - 777 |
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| Format: | Journal Article |
| Language: | English |
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Cuddalore
Journal of Pharmaceutical Sciences and Research
01-03-2019
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| Abstract | [...]nuclei quality and chromosomes spreads were examined by using microscope. [...]the hybridization is affected by many important factors that have a fundamental role in the hybridization process such as temperature, duration of the hybridization time, the activity of the post-hybridization washes, pH, formamide, and salt concentration. [...]this translocation was also detected in B cells of patients with non-Hodgkin's lymphoma (11). [...]using FISH technique can be very helpful in detection of translocation t(14;18)(q32;q21) in the Pfeiffer cell line that plays significant role in the diagnosis of DLBCL and can be used to examine therapy response and level of remaining disease. |
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| AbstractList | [...]nuclei quality and chromosomes spreads were examined by using microscope. [...]the hybridization is affected by many important factors that have a fundamental role in the hybridization process such as temperature, duration of the hybridization time, the activity of the post-hybridization washes, pH, formamide, and salt concentration. [...]this translocation was also detected in B cells of patients with non-Hodgkin's lymphoma (11). [...]using FISH technique can be very helpful in detection of translocation t(14;18)(q32;q21) in the Pfeiffer cell line that plays significant role in the diagnosis of DLBCL and can be used to examine therapy response and level of remaining disease. |
| Author | Al-Raheem, Zainab Saad Ghafil |
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| Snippet | [...]nuclei quality and chromosomes spreads were examined by using microscope. [...]the hybridization is affected by many important factors that have a... |
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| SubjectTerms | Alcohol Bone marrow Chromosomes Cytogenetics Deoxyribonucleic acid DNA Gene expression Genomics Hybridization Lymphoma Paints |
| Title | Identification of chromosomal-related abnormalities in Pfeiffer cell line using Fluorescence in situ hybridization |
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