Identification of chromosomal-related abnormalities in Pfeiffer cell line using Fluorescence in situ hybridization

[...]nuclei quality and chromosomes spreads were examined by using microscope. [...]the hybridization is affected by many important factors that have a fundamental role in the hybridization process such as temperature, duration of the hybridization time, the activity of the post-hybridization washes...

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Bibliographic Details
Published in:	Journal of pharmaceutical sciences and research Vol. 11; no. 3; pp. 774 - 777
Main Author:	Al-Raheem, Zainab Saad Ghafil
Format:	Journal Article
Language:	English
Published:	Cuddalore Journal of Pharmaceutical Sciences and Research 01-03-2019
Subjects:	Alcohol Bone marrow Chromosomes Cytogenetics Deoxyribonucleic acid DNA Gene expression Genomics Hybridization Lymphoma Paints
Online Access:	Get full text
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Summary:	[...]nuclei quality and chromosomes spreads were examined by using microscope. [...]the hybridization is affected by many important factors that have a fundamental role in the hybridization process such as temperature, duration of the hybridization time, the activity of the post-hybridization washes, pH, formamide, and salt concentration. [...]this translocation was also detected in B cells of patients with non-Hodgkin's lymphoma (11). [...]using FISH technique can be very helpful in detection of translocation t(14;18)(q32;q21) in the Pfeiffer cell line that plays significant role in the diagnosis of DLBCL and can be used to examine therapy response and level of remaining disease.
ISSN:	0975-1459