Chemotactically directed redistribution of [alpha]-actinin precedes morphological polarization and reversal of polarity in human polymorphonuclear leucocytes (PMNs)
We have determined the temporal and spatial relationship between cell polarization and alpha-actinin localization by analysing the redistribution of alpha-actinin and F-actin in spherical PMNs developing polarity and in polarized cells reversing polarity following localized stimulation with chemotac...
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| Published in: | European journal of cell biology Vol. 82; no. 2; p. 93 |
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| Main Authors: | , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Stuttgart
Elsevier Science Ltd
01-02-2003
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| Online Access: | Get full text |
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| Summary: | We have determined the temporal and spatial relationship between cell polarization and alpha-actinin localization by analysing the redistribution of alpha-actinin and F-actin in spherical PMNs developing polarity and in polarized cells reversing polarity following localized stimulation with chemotactic peptide using micropipettes. Initially spherical PMNs develop a one-sided accumulation of alpha-actinin before lamellipodia enriched in alpha-actinin are formed. In polarized cells, alpha-actinin is concentrated at the leading front. When polarity is reversed, alpha-actinin redistribution to the uropod precedes reversal of morphological polarity and formation of new lamellipodia at the uropod. Later, lamellipodia enriched in F-actin and alpha-actinin develop at the former uropod to form a new front. The data document that redistribution of alpha-actinin is a very early event in the development of polarity, which precedes formation of lamellipodia. |
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| ISSN: | 0171-9335 1618-1298 |