GENETICS OF TISSUE-SPECIFIC ANTHOCYANIN PIGMENTATION IN CARROT

GENETICS OF TISSUE-SPECIFIC ANTHOCYANIN PIGMENTATION IN CARROT

Purple carrots can accumulate anthocyanins in different root and leaf tissues. The consumption of these pigments may reduce the risk of cardiovascular disease, diabetes, arthritis and cancer, mainly due to their anti-oxidant and anti-inflammatory activities. In this study the genetic basis of anthoc...

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Bibliographic Details
Published in:BAG. Journal of basic and applied genetics Vol. 28; p. 126
Main Authors: Bannoud, F, Ellison, S, Fanzone, M, Senalik, D, Horejsi, T, Simon, P, Cavagnaro, P
Format: Journal Article
Language:Spanish
Published: Buenos Aires Sociedad Argentina de Genetica 01-01-2017
Subjects:
Anthocyanins
Arthritis
Association analysis
Cancer
Cardiovascular diseases
Diabetes mellitus
Gene expression
Gene mapping
Genotyping
High-performance liquid chromatography
Inflammation
Leaves
Linkage analysis
Phloem
Pigmentation
Pigments
Quantitative trait loci
Ribonucleic acid
RNA
Xylem
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Summary:Purple carrots can accumulate anthocyanins in different root and leaf tissues. The consumption of these pigments may reduce the risk of cardiovascular disease, diabetes, arthritis and cancer, mainly due to their anti-oxidant and anti-inflammatory activities. In this study the genetic basis of anthocyanin accumulation in different tissues was investigated. An F2 population (N= 254) segregating for anthocyanin accumulation was visually phenotyped by scoring anthocyanin pigmentation in the root xylem and phloem and in leaf petioles. Additionally, the content of 5 anthocyanin pigments in the root xylem and phloem, independently, was estimated by HPLC analysis. A linkage map was constructed using 1014 Genotyping by Sequencing (GBS) markers, distributed across 9 linkage groups (LG), which covered 910 cM of total map length, with LGs ranging from 74.2 cM (Chr 5) to 124 cM (Chr 1). Genome wide association analysis in this F2 identified regions associated with tissue-specific anthocyanin pigmentation in carrot root. In addition, a region spanning ~1.5 Mbp of Chr 3 was significantly associated with anthocyanin accumulation in leaf petioles and the root xylem, suggesting that the same region conditions pigmentation in both tissues. Several regions on Chr 3 were significantly associated with phloem pigmentation, suggesting multiple loci conditioning this trait. Ongoing QTL mapping and transcriptome analysis by RNA-Seq in purple and non-purple phloem tissues may help to further delimit the chromosome regions associated with phloem pigmentation and find candidate genes for
ISSN:1666-0390
1852-6233