Expression of [alpha]-taxilin in the murine gastrointestinal tract: potential implication in cell proliferation

• Published in: Histochemistry and cell biology Vol. 141; no. 2; p. 165
• Main Authors: Horii, Yukimi, Sakane, Hiroshi, Nogami, Satoru, Ohtomo, Natsuko, Tomiya, Tomoaki, Shirataki, Hiromichi
• Format: Journal Article
• Language: English
• Published: New York Springer Nature B.V 01-02-2014
• Subjects: Cellular biology; Digestive system; Immunohistochemistry; Proteins; Tumors
• ISSN: 0948-6143; 1432-119X
• DOI: 10.1007/s00418-013-1147-0

[alpha]-Taxilin, a binding partner of the syntaxin family, is a candidate tumor marker. To gain insight into the physiological role of [alpha]-taxilin in normal tissues, we examined [alpha]-taxilin expression by Western blot and performed immunochemical analysis in the murine gastrointestinal tract where cell renewal vigorously occurs. [alpha]-Taxilin was expressed in the majority of the gastrointestinal tract and was prominently expressed in epithelial cells positive for Ki-67, a marker of actively proliferating cells. In the small intestine, [alpha]-taxilin was expressed in transient-amplifying cells and crypt base columnar cells intercalated among Paneth cells. In the corpus and antrum of the stomach, [alpha]-taxilin was expressed in cells localized in the lower pit and at the gland, respectively, but not in parietal or zymogenic cells. During development of the small intestine, [alpha]-taxilin was expressed in Ki-67-positive regions. Inhibition of cell proliferation by suppression of the Notch cascade using a γ-secretase inhibitor led to a decrease in [alpha]-taxilin- and Ki-67-positive cells in the stomach. These results suggest that expression of [alpha]-taxilin is regulated in parallel with cell proliferation in the murine gastrointestinal tract.[PUBLICATION ABSTRACT]