Detection and Structural Elucidation of Esterified Oxylipids in Human Synovial Fluid by Electrospray Ionization-Fourier Transform Ion-Cyclotron Mass Spectrometry and Liquid Chromatography-Ion Trap-MS^sup 3^: Detection of Esterified Hydroxylated Docosapentaenoic Acid Containing Phospholipids

• Published in: Analytical chemistry (Washington) Vol. 85; no. 12; p. 6003
• Main Authors: Jónasdóttir, Hulda S, Nicolardi, Simone, Jonker, Willem, Derks, Rico, Palmblad, Magnus, Ioan-Facsinay, Andreea, Toes, René, van der Burgt, Yuri E M, Deelder, André M, Mayboroda, Oleg A, Giera, Martin
• Format: Journal Article
• Language: English
• Published: Washington American Chemical Society 18-06-2013
• Subjects: Body fluids; Chromatography; Fourier transforms; Lipids; Mass spectrometry; Rheumatoid arthritis; Statistical analysis
• ISSN: 0003-2700; 1520-6882

Here, we present the application of a cross-platform approach, combining rapid direct infusion high-resolution/accurate mass electrospray ionization Fourier transform ion-cyclotron mass spectrometry (ESI-FTICRMS) with in-depth data-dependent LC-MS... and LC-MS... analysis for lipid profiling. The analytical approach as well as the subsequent data handling is described. The method was applied to human synovial fluid samples from osteo- and rheumatoid arthritis patients. Multivariate statistical analysis revealed esterified oxylipids as molecular features in a subset of the patient samples. Employing LC-MS... and LC-MS... analysis of these species, we were able to clarify the hypothesized lipid structures initially based on the accurate mass measurements performed on the ESI-FTICRMS platform. LC-MS... analysis of intact esterified oxy-lipids and LC-MS... analysis of the hydrolysis products allowed for the detection of positional isomers. The approach led to the structural elucidation of hydroxylated docosapentaenoic acid-containing diacyl-phosphatidylcholine type phospholipids in human synovial fluid. (ProQuest: ... denotes formulae/symbols omitted.)