Development of a Novel Vector System for Programmed Cell Lysis inEscherichia coli

• Published in: Journal of microbiology and biotechnology pp. 1162 - 1168
• Main Authors: 윤지애, JIHYE PARK, NANJOO PARK, SEOWON KANG, 유상렬
• Format: Journal Article
• Language: English
• Published: 한국미생물·생명공학회 01-07-2007
• Subjects: 생물학
• ISSN: 1017-7825

protein production, Escherichia coli is unsuitable for massivescreening of recombinant clones, owing to its poor secretionof proteins. A vector system containing T4 holin and T7lysozyme genes under the control of the ptsG promoterderivative that is inducible in the absence of glucose wasdeveloped for programmed cell lysis of E. coli. Because E.coli harboring the vector grows wel in the presence ofglucose, but is lysed upon glucose exhaustion, the activity ofE. coli can be monitored easilywithout an additional step for cel disruption after the foreigngene is expressed sufficiently with an apropriate concentrationof glucose. The effectiveness of the vector was demonstratedby efficient screening of the amylase gene from a Bacilussubtilis genomic library. This vector system is expected toprovide a more efficient and economic screening of bioactiveproducts from DNA libraries in large quantities. KCI Citation Count: 3