Isolation and Structure Elucidation, Molecular Docking Studies of Screlotiumol from Soil Borne Fungi Screlotium rolfsii and their Reversal of Multidrug Resistance in Mouse Lymphoma Cells

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 17; no. 4; pp. 2083 - 2087
• Main Authors: Ahmad, Bashir, Rizwan, Muhammad, Rauf, Abdur, Raza, Muslim, Azam, Sadiq, Bashir, Shumaila, Molnar, Joseph, Csonka, Akos, Szabo, Diana
• Format: Journal Article
• Language: Korean
• Published: 2016
• Subjects: molecular docking; Screlotium rolfsii; Soil borne fungi; MDR; screlotiumol 1
• ISSN: 1513-7368; 2476-762X

A new compound namely (13-(3,3-dihydroxypropyl)-1,6-dihydroxy-3,4-dihydro-1H-isochromen-8(5H)-one (1) was isolated from an ethyl acetate extract of the borne fungi Screlotium rolfsii. Its chemical structure was elucidated by spectroscopic analysis. Screlotiumol 1 were evaluated for their effects on the reversion of multidrug resistant (MDR) mediated by P-glycoprotein (P-gp) of the soil borne fungi. The multidrug resistant P-glycoprotein is a target for chemotherapeutic drugs in cancer cells. In the present study rhodamine-123 exclusion screening test on human mdr1 gene transfected mouse gene transfected L5178 and L5178Y mouse T-cell lymphoma which showed excellent MDR reversing effect in a dose dependent manner against mouse T-lymphoma cell line. Moreover, molecular docking studies of compound-1 also showed better results as compared with the standard. Therefore the preliminary results obtained from this study suggest that screlotiumol 1 could be used as a potential agent for the treatment of cancer.