Release of Renal Dipeptidase from Rabbit Renal Proximal Tubules and Its Inhibition by Gentamicin

Effects of several durgs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 11) release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control exper...

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Bibliographic Details
Published in:	Archives of pharmacal research Vol. 22; no. 4; pp. 367 - 371
Main Authors:	Kang, Bok-Yun, We, Jeoung-Soon, Choi, Kyong, Lee, Hwanghee-Blaise, Han, Ho-Jae, Park, Haeng-Soon
Format:	Journal Article
Language:	Korean
Published:	1999
Subjects:	Proximal tubule Renal dipeptidase (RDPase) Gentamicin Glycosylphosphatidylinositol (GPI)-protein Phosphatidyloinositol specific phospholipase C (PI-PLC)
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Summary:	Effects of several durgs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 11) release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control experiment which was confirmed by Western blotting. RDPase was also released form cisplatin, lipopolysaccardie (LPS), and indomethacin-treated tubules. Gentamicin inhibited RDPase release in a concentration-dependent manner. This RDPase release system may not be a general model to screen nephrotoxicity but could be a useful source of RDPase purification in a simple and inexpensive way.
Bibliography:	KISTI1.1003/JNL.JAKO199911919476531
ISSN:	0253-6269 1976-3786