Teste de DNA para verificação de parentesco em cães: avaliação do método não automatizado com o auxílio do primer CMR S

• Published in: Arquivo brasileiro de medicina veterinária e zootecnia Vol. 54; no. 5
• Main Authors: Oliveira, P.F.(UFMG Escola de Veterinária), Oliveira, D.A.A.(UFMG Escola de Veterinária), Teixeira, C.S.(UFMG Escola de Veterinária), Velloso, A.P.S.(UFMG Escola de Veterinária), Coelho, E.G.A.(UFMG Escola de Veterinária), Rodrigues, S.G.(UFMG Escola de Veterinária), Alves, C.(UFMG Escola de Veterinária)
• Format: Journal Article
• Language: Portuguese
• Published: Universidade Federal de Minas Gerais, Escola de Veterinária 2002
• Subjects: Cão; DNA; Dog; parentage tests; teste de verificação de parentesco
• ISSN: 0102-0935; 1678-4162

To evaluate the precision of the DNA tests using the non-automatized technique for individual identification and parentage tests, 105 Rottweiler dogs were studied using the primer CMR S. The sample was composed of 39 animals belonging to 11 complete families and their progenies, and 66 non related individuals until the second generation, derived from kennels located in the states of Minas Gerais and São Paulo. The CMR S primer was used for the Polimerase Chain Reaction (PCR). The results showed the inefficiency of the technique, even when analyzed through the automated gel analysis system. Also showed the impossibility of its commercial use due to the fact of does not permit the storage of data for subsequent use.