DETECTION OF YERSINIA RUCKERI BY PCR IN RAINBOW TROUT (ONCORHYNCHUSMYKISS- WALBAUM) HATCHERY FARMS IN THE WEST OF TURKEY

ABSTRACT The presence of Yersinia ruckeri in rainbow trout hatchery farms was investigated by PCR Assay. Twenty-five rainbow trout, suspected of Enteric Redmouth Disease, were examined in this study. Yersinia ruckeri strains were identified from 8 out of 25 samples (32%). Ten of 25 (40%) rainbow tro...

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Published in:İstanbul Üniversitesi Veteriner Fakültesi dergisi
Main Authors: KIRKAN, Şükrü, GÖKSOY, Ergün Ömer, TEKBIYIK, Serten, KAYA, Osman
Format: Journal Article
Language:Turkish
Published: İstanbul Üniversitesi 21-12-2012
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Summary:ABSTRACT The presence of Yersinia ruckeri in rainbow trout hatchery farms was investigated by PCR Assay. Twenty-five rainbow trout, suspected of Enteric Redmouth Disease, were examined in this study. Yersinia ruckeri strains were identified from 8 out of 25 samples (32%). Ten of 25 (40%) rainbow trout samples showed positive reactions for Yersinia ruckeri in the PCR. All PCR positive samples have shown specific amplification bands of the 575-bp fragment for Yersinia ruckeri. Key Words: Yersinia ruckeri, Rainbow trout, PCR, diagnosis.   TÜRKİYE'NİN BATISINDAKİ GÖKKUŞAĞI ALABALIK YETİŞTİRME ÇİFTLİKLERİNDE YERSİNİA RUCKERİ'NİN PZR KULLANILARAK TANIMLANMASI ÖZET Bu araştırmada, Gökkuşağı Alabalığı çiftliklerindeki Yersinia ruckeri varlığı Polimeraz Zincir Reaksiyonu (PZR) metodu ile incelendi. Bu çalışmada Kızıl Ağız Hastalığı §şüpheli 25 adet Gökkuşağı Alabalığı materyal olarak kullanıldı. 25 örneğin 8 (%32)'inden Yersinia ruckeri identifiye edildi. 25 örneğin 10'unda PZR'da Yersinia ruckeri yönünden pozitiflik saptandı. PZR pozitif ürünlerin hepsinde, Yersinia ruckeri için spesifik 575 bp uzunluğunda bantlar elde edildi. Anahtar ...
Bibliography:http://www.journals.istanbul.edu.tr/iuvfd/article/view/1019019322
10.16988/iuvfd.21905
ISSN:2148-8320
0250-2836