Nanofiber poly ether sulfone as adipose derived stem cell holding scaffold with or without growth factor on wound healing in rat

• Published in: Majallah-i Danishkadah-'i Pizishki Vol. 74; no. 12; pp. 843 - 851
• Main Authors: Mohammd Javad Fatemi, Shirin Chehroudi, Tooran Bagheri, Sahar Saleh, Amir Atashi, Mohsen Saberi, Seyed Aboozar Hoseini, Shirin Araghi
• Format: Journal Article
• Language: Persian
• Published: Tehran University of Medical Sciences 01-03-2017
• Subjects: growth factor; nanofibers; polyether sulfone; stem cells; tissue scaffolds
• ISSN: 1683-1764; 1735-7322

Background: Acute and chronic wound healing has always been problematic. Stem cells with or without the scaffold carrying these cells have been proposed as new methods in the treatment of wounds. In this case study we have tried to examine the effect of scaffold made of polyether sulfone (PES) alone, with stem cells and along with stem cell and growth factor on wound healing in rats. Methods: This experimental study was conducted in Animal Laboratory of Hazrat Fatemeh Hospital in 2012. In this study, 48 rats were randomly divided into four groups. A wound created on the back of each rat at the size of 3×3 cm. The surface of the wound in the first group is covered with PES seeded with adipose-derived stem cell (ASC) and growth factor (GF), in the second group with polyether Sulfone seeded with ASC, in the third group only with PEWS, and in the fourth group (control) with Vaseline gauze. On 20th and 35th days, the surface of the wound was assessed by photography in order to understand the process of healing. In addition, on days 20 and 45, the histopathology characteristics of the samples were studied with a biopsy of the wounds. Results: The Results of wound healing in the control group was better than the other groups and its statistical difference between others was meaningful. (P=0.008, P=0.013, P=0.001) On day 20, by examining histopathological characteristics including epithelialization, the number of inflammatory cells, the amount of angiogenesis and collagen synthesis in control group, we gained better results. (P=0.000), But on day 45, the results in different parameters were not equal. Conclusion: polyether sulfone scaffold alone or with adipose-derived stem cells couldn't improve the process of wound healing. Also adding vascular endothelial growth factor (VEGF) did not change the results significantly.