A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis

A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis

Human respiratory syncytial virus (HRSV) causes severe infections among children and immunocompromised patients. We compared HRSV infections among Haematopoietic Stem Cell Transplant program (HSCT) patients and children using direct immunofluorescence (DFA), point-of-care RSV Bio Easy® and a polymer...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz Vol. 108; no. 1; pp. 119 - 122
Main Authors: Luciana Peniche Moreira, Aripuanã Sakurada Aranha Watanabe, Emerson Carraro, Ellen Ricci Monteiro da Silva, Sandra Baltazar Guatura, Celso Granato, Nancy Junqueira Bellei
Format: Journal Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 01-02-2013
Subjects:
comparison methods
human stem cell transplant patients
respiratory syncytial virus
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Summary:Human respiratory syncytial virus (HRSV) causes severe infections among children and immunocompromised patients. We compared HRSV infections among Haematopoietic Stem Cell Transplant program (HSCT) patients and children using direct immunofluorescence (DFA), point-of-care RSV Bio Easy® and a polymerase chain reaction (PCR) assay. Overall, 102 samples from HSCT patients and 128 from children obtained positivity rate of 18.6% and 14.1% respectively. PCR sensitivity was highest mainly on samples collected after five days of symptoms onset. A combination of both DFA and reverse transcriptase-PCR methods for HSCT high-risk patients is the best diagnostic flow for HRSV diagnosis among these patients.
ISSN:1678-8060
DOI:10.1590/S0074-02762013000100021