Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan - Iran

• Published in: Iranian journal of parasitology Vol. 8; no. 3
• Main Authors: Haleh Hanifian, Kambiz Diba, Khosrow Hazrati Tappeh, Habib Mohammadzadeh, Rahim Mahmoudlou
• Format: Journal Article
• Language: English
• Published: Tehran University of Medical Sciences 01-09-2013
• Subjects: Hydatid cyst; ITS1; RFLP-PCR; Strain
• ISSN: 1735-7020; 2008-238X

Background: The aim of this study was DNA extraction from protosco-lecses of Echinococcus granulosus and identification of these strains in West- Azerbaijan Province, north western Iran. Methods: Thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. To investigate the genetic variation of the isolates, after DNA extraction by Glass beads-phenol chloroform method; PCR-RLFP analysis of rDNA-ITS1 was performed using three different re-striction enzymes of Taq 1, Rsa 1 and Alu 1. Result: Amplified PCR products for all isolates were 1000bp band which is expected band in sheep strains (G1-G3 complex). The results of RFLP analy-sis also were the same for all isolates. PCR-RFLP patterns restriction en-zymes were identical as follows, Rsa1 bands under UV showed two bands approximately 655bp and 345bp. Alu1 bands were as follows: two approx-imately 800bp and 200bp and Taq1 did not cut any region and bands were approximately 1000 bp in all samples. Conclusions: Based on PCR-RFLP patterns of ITS1 fragment produced with endonucleases enzyme digestion in animal isolates, it can be concluded that a single strain of E. granulosus (sheep strain or G1-G3 complex) is domi-nant genotype in this province.