电针对神经病理性疼痛大鼠及其脊髓EAAs含量的影响
目的：观察电针对神经病理性疼痛大鼠痛阈及脊髓兴奋性氨基酸含量的影响,探讨电针治疗神经病理性疼痛的机制.方法：SD大鼠随机分为对照组、假模型组和造模组,采用大鼠坐骨神经分支选择性损伤（Spared Nerve Injury, SNI） 模型,造模组SNI 术后选取造模成功的大鼠再随机分为模型组、电针组、假电针组.分别于SNI术前、术后第7 天及第9 天、第6 次治疗后测定大鼠的机械痛阈和热痛阈.造模后第l0 天开始电针环跳穴与委中穴,观察其对大鼠机械痛阈和热痛阈的影响,并通过脊髓微透析技术,应用柱前衍生法＋HPLC荧光检测法检测大鼠脊髓兴奋性氨基酸的含量.结果：SNI 手术可以显著降低大鼠机械痛...
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| Published in: | 针灸推拿医学：英文版 Vol. 9; no. 2; pp. 73 - 78 |
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| Main Author: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2011
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| Online Access: | Get full text |
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| Summary: | 目的：观察电针对神经病理性疼痛大鼠痛阈及脊髓兴奋性氨基酸含量的影响,探讨电针治疗神经病理性疼痛的机制.方法：SD大鼠随机分为对照组、假模型组和造模组,采用大鼠坐骨神经分支选择性损伤（Spared Nerve Injury, SNI） 模型,造模组SNI 术后选取造模成功的大鼠再随机分为模型组、电针组、假电针组.分别于SNI术前、术后第7 天及第9 天、第6 次治疗后测定大鼠的机械痛阈和热痛阈.造模后第l0 天开始电针环跳穴与委中穴,观察其对大鼠机械痛阈和热痛阈的影响,并通过脊髓微透析技术,应用柱前衍生法＋HPLC荧光检测法检测大鼠脊髓兴奋性氨基酸的含量.结果：SNI 手术可以显著降低大鼠机械痛阈,模型组大鼠脊髓微透析液中谷氨酸（Glutamate, Glu ）和天门冬氨酸（Aspartic Acid, Asp ）的含量较同时段对照组、假模型组有显著升高（P〈0.05） ;电针组、假电针组脊髓微透析液中Glu和Asp 含量与同时段模型组比较明显减少,差异具有统计学意义（P〈0.05）（假电针组第2 时段Glu除外）,并且电针可以显著减轻SNI 大鼠的机械痛敏状态.结论：电针治疗神经病理性疼痛可能与降低脊髓兴奋性氨基酸含量有关. |
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| Bibliography: | Pain; Pain Threshold; Chromatography, High Pressure Liquid; Excitatory Amino Acids; Electroacupuncture; Neuralgia; Rats Objective： To observe the impact of electroacupuncture on the pain threshold and the content of excitatory amino acids in the spinal cord of the rats with neuropathic pain to discuss the mechanism of electroacupuncture in treating neuropathic pain. Methods： The rats were randomly divided into a control group （n=10）, a sham surgery group （n=10） and a surgery group （n=30） to take spared nerve injury （SNI） surgery of sciatic nerve. The successful SNI model rats in the surgery group were randomly divided into a model group （n=10）, an electroacupuncture group （n=10） and a sham electroacupuncture group （n=10）. The mechanical pain threshold （MPT） and heat pain threshold （HPT） were measured on the preoperative day, postoperative day 7, postoperative day 9 and the day after the sixth acupuncture treatment. The electroacupuncture was carried out at Huantiao （GB 30） and Weizhong （BL 40） starting from the postoperative day 10 to reflect the impact on the mechanical pain threshold and heat pain threshold. The pre-column deriverization and HPLC fluorescence detection as well as microdialysis method were adopted to test the content of EAAs in spinal cord of rats. Results： The SNI surgery decreased the mechanical pain threshold of rats significantly. The glutamate （Glu） and aspartic acid （Asp） in the microdialysis solution of rats in the model group were higher than in the control group and sham surgery group at the same time point （P〈0.05）. The contents of Glu and Asp in the microdialysis solution of rats in the electroacupuncture group and sham electroacupuncture group decreased significantly （P〈0.05）, comparing with model group at the same time point （except the Glu of the sham electroacupuncture group at the second time point）. The electroacupuncture reduced the mechanical allodynia markedly. Conclusion： The mechanism of electroacupuncture treating neuropathic pain may relate with the decrease of excitatory amino acids in spinal cord. 31-1908/R Li Cuixian , Yan Liping, Yi Jianliang, Ma Cheng （ Key Laboratory of Acupuncture of Jiangsu, Nangjing University of Traditional Chinese Medicine, Nanjing 210029 P. R. China） |
| ISSN: | 1672-3597 1993-0399 |