Analysis of the meiosis in the F1 hybrids of Longiflorum × Asiatic （LA） of lilies （Lilium） using genomic in situ hybridization

• Published in: Journal of genetics and genomics Vol. 35; no. 11; pp. 687 - 695
• Main Author: Shujun Zhou Munikote S. Ramanna Richard G.E Visser Jaap M. van Tuyl
• Format: Journal Article
• Language: Chinese
• Published: 2008
• Subjects: 减数分裂; 分析方法; 原位杂交; 基因组; 百合花; 麝香
• ISSN: 1673-8527

Longiflorum and Asiatic lilies of the genus Lilium of the family Liliaceae are two important groups of modem lily cultivars. One of the main trends of lily breeding is to realize introgression between these groups. With cut style pollination and embryo rescue, distant hybrids between the two groups have been obtained. However, the FI hybrids are highly sterile or some of them could produce a small number of 2n gametes, and their BC1 progenies are usually triploids. Dutch lily breeders have selected many cultivars from these BC1 progenies based on their variation. It is presumably suggested that such variation could be caused by intergenomic recombination and abnormal meiosis during gamete formation in F1 hybrids of Longiflorum × Asiatic （LA） hybrids in Lilium. Therefore, the meiotic process of ten F1 LA hybrids was cytologically investigated using genomic in situ hybridization and traditional cytological methods in the present research. The results showed that： at metaphase I, the homoeologous chromosome pair