Development of a DNA probe and PCR assay for the detection of Actinobacillus pleuropneumoniae

A DNA probe was created for the detection of Actinobacillus pleuropneumoniae DNA from a genomic library of BamHI digested A. pleuropneumoniae serotype 2 (27089) DNA cloned in the BamHI site of the plasmid vector pTZ18U. The probe, pTA4, contains a 3.1 Kb insert of A. pleuropneumoniae and is specific...

Full description

Saved in:

Bibliographic Details
Main Author:	Thornton, Kathryn Ann
Format:	Dissertation
Language:	English
Subjects:	biology, microbiology biology, molecular biology, veterinary science
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A DNA probe was created for the detection of Actinobacillus pleuropneumoniae DNA from a genomic library of BamHI digested A. pleuropneumoniae serotype 2 (27089) DNA cloned in the BamHI site of the plasmid vector pTZ18U. The probe, pTA4, contains a 3.1 Kb insert of A. pleuropneumoniae and is specific in its affinity for all serotypes of A. pleuropneumoniae analyzed. DNA sequences were obtained from the 3.1 Kb insert. Five primers were designed for use in the polymerase chain reaction. DNA amplification of purified DNA from all serotypes A. pleuropneumoniae analyzed was achieved. Preliminary evidence indicates that amplification of Haemophilus parasuis, Bordetella bronchiseptica, Escherichia coli, and leukocytic swine DNA is unlikely and that the amplification of P. multocida DNA could be eliminated with two specific combination of the five primers.
Bibliography:	Source: Masters Abstracts International, Volume: 33-05, page: 1459. Supervisor: John N. Berg.