Cryptic species of Paracoccidioides brasiliensis : impact on paracoccidioidomycosis immunodiagnosis
We aimed to evaluate whether the occurrence of cryptic species of Paracoccidioides brasiliensis , S1, PS2, PS3 and Paracoccidioides lutzii , has implications in the immunodiagnosis of paracoccidioidomycosis (PCM). Small quantities of the antigen gp43 were found in culture filtrates of P. lutzii stra...
Saved in:
Published in: | Memórias do Instituto Oswaldo Cruz Vol. 108; no. 5 |
---|---|
Main Authors: | , , , , , , , |
Format: | Journal Article |
Language: | English |
Published: |
Fundação Oswaldo Cruz, Fiocruz
15-01-2014
|
Subjects: | |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | We aimed to evaluate whether the occurrence of cryptic species of
Paracoccidioides brasiliensis , S1, PS2, PS3 and Paracoccidioides
lutzii , has implications in the immunodiagnosis of
paracoccidioidomycosis (PCM). Small quantities of the antigen gp43 were
found in culture filtrates of P. lutzii strains and this molecule
appeared to be more variable within P. lutzii because the
synonymous-nonsynonymous mutation rate was lower, indicating an
evolutionary process different from that of the remaining genotypes.
The production of gp43 also varied between isolates belonging to the
same species, indicating that speciation events are important, but not
sufficient to fully explain the diversity in the production of this
antigen. The culture filtrate antigen AgEpm83, which was obtained from
a PS3 isolate, showed large quantities of gp43 and reactivity by
immunodiffusion assays, similar to the standard antigen (AgB-339) from
an S1 isolate. Furthermore, AgEpm83 was capable of serologically
differentiating five serum samples from patients from the Botucatu and
Jundiaí regions. These patients had confirmed PCM but, were
non-reactive to the standard antigen, thus demonstrating an alternative
for serological diagnosis in regions in which S1 and PS2 occur. We also
emphasise that it is not advisable to use a single antigen preparation
to diagnose PCM, a disease that is caused by highly diverse pathogens. |
---|---|
ISSN: | 1678-8060 |