Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivaxv

The PfCLAG9 has been extensively studied because their immunogenicity. Thereby, the gene product is important for therapeutics interventions and a potential vaccine candidate. Antibodies against synthetic peptides corresponding to selected sequences of the Plasmodium falciparum antigen PfCLAG9 were...

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Bibliographic Details
Published in:	Memórias do Instituto Oswaldo Cruz Vol. 108; no. 1
Main Authors:	Costa, Joana D'Arc Neves, Zanchi, Fernando Berton, Rodrigues, Francisco Lurdevanhe da Silva, Honda, Eduardo Rezende, Katsuragawa, Tony Hiroschi, Pereira, Dhélio Batista, Taborda, Roger Lafontaine Mesquita, Tada, Mauro Shugiro, Ferreira, Ricardo de Godoi Mattos, Pereira-da-Silva, Luiz Hildebrando
Format:	Journal Article
Language:	English
Published:	Fundação Oswaldo Cruz, Fiocruz 13-03-2013
Subjects:	malaria - immunity - Amazon - cross-reaction
Online Access:	Get full text
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Summary:	The PfCLAG9 has been extensively studied because their immunogenicity. Thereby, the gene product is important for therapeutics interventions and a potential vaccine candidate. Antibodies against synthetic peptides corresponding to selected sequences of the Plasmodium falciparum antigen PfCLAG9 were found in sera of falciparum malaria patients from Rondônia, in the Brazilian Amazon. Much higher antibody titres were found in semi-immune and immune asymptomatic parasite carriers than in subjects suffering clinical infections, corroborating original findings in Papua Guinea. However, sera of Plasmodium vivax patients from the same Amazon area, in particular from asymptomatic vivax parasite carriers, reacted strongly with the same peptides. Bioinformatic analyses revealed regions of similarity between P. falciparum Pfclag9 and the P. vivax ortholog Pvclag7. Indirect fluorescent microscopy analysis showed that antibodies against PfCLAG9 peptides elicited in BALB/c mice react with human red blood cells (RBCs) infected with both P. falciparum and P. vivax parasites. The patterns of reactivity on the surface of the parasitised RBCs are very similar. The present observations support previous findings that PfClaglag 9 may be a target of protective immune responses and raises the possibility that the cross reactive antibodies to PvCLAG7 in mixed infections play a role in regulate the fate of Plasmodium mixed infections.
ISSN:	1678-8060