Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol

• Published in: Electronic Journal of Biotechnology Vol. 13; no. 6
• Main Authors: Gedikli, Serap, Aytar, Pinar, Ünal, Arzu, Yamaç, Mustafa, Çabuk, Ahmet, Kolankaya, Nazif
• Format: Journal Article
• Language: English
• Published: Universidad Católica de Valparaíso 11-04-2011
• Subjects: 2,4,5-trichlorophenol, basidiomycetes, dechlorination, inducer, lacasse
• EISSN: 0717-3458

Lacasse is one of the extracellular enzymes excreted from white and
brown rot fungi, which is involved in ligninolysis. In the present
study, the effects of the addition of lacasse inducers to the medium on
enhancement of enzyme production under conditions of submerged
fermentation were researched. At first, a culture medium was selected
suitable for lacasse production. To increase the production of lacasse
using different inducers and to examine the ability of dechlorination,
this article focuses on screen lacasse activity of 21 basidiomycetes
isolates grown in five culture media. All inducers evaluated influenced
lacasse activity positively except for gallic acid, mannitol, and malt
extract for studied isolates. Our findings showed that lacasse activity
of Trametes versicolor ATCC (200801) when it was induced with wheat
bran reached up to 29.08 U ml-1 and was examined the ability of
dechlorination of 2,4,5-trichlorophenol (2,4,5-TCP). The parameters
including pH, initial substrate concentration, amount of enzyme, period
of reaction, and temperature were tested for dechlorination process.
Correlation between oxygen consumption and dechlorination processes
under the determined optimum conditions was analyzed. Toxicity of
2,4,5-TCP before and after enzymatic treatment was evaluated by
Microtox test. The results demonstrated that toxicity of intermediates
formed 2,4,5-TCP did not change.