DIMETILACETAMIDA ASSOCIADA OU NÃO AO GLICEROL PARA CRIOPRESERVAÇÃO DE SÊMEN OVINO
Resumo O objetivo do presente estudo foi testar a dimetilacetamida (DMA) em diferentes concentrações, associada ou não ao glicerol (GL), sobre a viabilidade espermática do sêmen ovino congelado. Foram utilizados 10 ejaculados de dois carneiros adultos da raça Santa Inês. Os ejaculados foram dividido...
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| Published in: | Ciência animal brasileira Vol. 19 |
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2018
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| Abstract | Resumo O objetivo do presente estudo foi testar a dimetilacetamida (DMA) em diferentes concentrações, associada ou não ao glicerol (GL), sobre a viabilidade espermática do sêmen ovino congelado. Foram utilizados 10 ejaculados de dois carneiros adultos da raça Santa Inês. Os ejaculados foram divididos em sete grupos experimentais, respeitando o limite máximo de 5% de DMA, sendo eles: GL6%, DMA3%, GL5%+DMA1%, GL4%+DMA2%, GL3%+DMA3%, GL2%+DMA4%, GL1%+DMA5%. Os espermatozoides criopreservados nos diferentes tratamentos foram analisados quanto à cinética subjetiva, integridade estrutural da membrana plasmática (EOS), integridade funcional da membrana plasmática (CO) e morfologia espermática, observando defeitos totais (DT) e defeitos maiores (DM). A motilidade total (MT) e a progressiva (MP) pós-descongelação nos grupos GL5%+DMA1%; GL4%+DMA2% e GL3%+DMA3%, foram semelhantes (P>0,05) ao tratamento controle (GL6%). Destes, o diluidor GL4%+DMA2% foi o único que promoveu a manutenção da MT e MP pós-descongelação, quando comparado com o sêmen in natura (P>0,05). Não foram observadas diferenças significativas (P>0,05) para os parâmetros de EOS, CO, DT e DM nos diferentes grupos avaliados. A dimetilacetamida associada ao glicerol mostrou-se eficaz na manutenção da viabilidade espermática em ovinos, avaliada pós-descongelação. Entretanto, foi observado efeito deletério da DMA nas concentrações mais elevadas ou quando não esteve associada ao glicerol. |
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| AbstractList | Resumo O objetivo do presente estudo foi testar a dimetilacetamida (DMA) em diferentes concentrações, associada ou não ao glicerol (GL), sobre a viabilidade espermática do sêmen ovino congelado. Foram utilizados 10 ejaculados de dois carneiros adultos da raça Santa Inês. Os ejaculados foram divididos em sete grupos experimentais, respeitando o limite máximo de 5% de DMA, sendo eles: GL6%, DMA3%, GL5%+DMA1%, GL4%+DMA2%, GL3%+DMA3%, GL2%+DMA4%, GL1%+DMA5%. Os espermatozoides criopreservados nos diferentes tratamentos foram analisados quanto à cinética subjetiva, integridade estrutural da membrana plasmática (EOS), integridade funcional da membrana plasmática (CO) e morfologia espermática, observando defeitos totais (DT) e defeitos maiores (DM). A motilidade total (MT) e a progressiva (MP) pós-descongelação nos grupos GL5%+DMA1%; GL4%+DMA2% e GL3%+DMA3%, foram semelhantes (P>0,05) ao tratamento controle (GL6%). Destes, o diluidor GL4%+DMA2% foi o único que promoveu a manutenção da MT e MP pós-descongelação, quando comparado com o sêmen in natura (P>0,05). Não foram observadas diferenças significativas (P>0,05) para os parâmetros de EOS, CO, DT e DM nos diferentes grupos avaliados. A dimetilacetamida associada ao glicerol mostrou-se eficaz na manutenção da viabilidade espermática em ovinos, avaliada pós-descongelação. Entretanto, foi observado efeito deletério da DMA nas concentrações mais elevadas ou quando não esteve associada ao glicerol. |
| Author | Menezes, Gabriel Felipe Oliveira Bittencourt, Rodrigo Freitas Santos, Elisiane Sateles Ribeiro Filho, Antônio de Lisboa Cardoso, Fernando de Lima Silva, Mariana Alves Andrade |
| AuthorAffiliation | Universidade Federal da Bahia |
| AuthorAffiliation_xml | – name: Universidade Federal da Bahia |
| Author_xml | – sequence: 1 givenname: Gabriel Felipe Oliveira surname: Menezes fullname: Menezes, Gabriel Felipe Oliveira organization: Universidade Federal da Bahia – sequence: 2 givenname: Rodrigo Freitas surname: Bittencourt fullname: Bittencourt, Rodrigo Freitas organization: Universidade Federal da Bahia – sequence: 3 givenname: Antônio de Lisboa surname: Ribeiro Filho fullname: Ribeiro Filho, Antônio de Lisboa organization: Universidade Federal da Bahia – sequence: 4 givenname: Fernando de Lima surname: Cardoso fullname: Cardoso, Fernando de Lima organization: Universidade Federal da Bahia – sequence: 5 givenname: Mariana Alves Andrade surname: Silva fullname: Silva, Mariana Alves Andrade organization: Universidade Federal da Bahia – sequence: 6 givenname: Elisiane Sateles surname: Santos fullname: Santos, Elisiane Sateles organization: Universidade Federal da Bahia |
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| DOI | 10.1590/1809-6891v19e-48026 |
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| Discipline | Agriculture |
| DocumentTitleAlternate | DIMETHYLACETAMIDE ASSOCIATED OR NOT TO GLYCEROL FOR CRIOPRESERVATION OF SHEEP SEMEN |
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| Keywords | ovino semen dimethylacetamide dimetilacetamida criopreservação cryopreservation sheep sêmen |
| Language | Portuguese |
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Castelo, TS; Alves, HM; Oliveira, IRS; Lima, GL; Peixoto, GCX; Bezerra, ACSD; Silva, AR |
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| Title | DIMETILACETAMIDA ASSOCIADA OU NÃO AO GLICEROL PARA CRIOPRESERVAÇÃO DE SÊMEN OVINO |
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