Essential oils from Argentinean native species reduce in vitro methane production

• Published in: RIA. Revista de investigaciones agropecuarias Vol. 44; no. 1; pp. 76 - 83
• Main Authors: Garcia, Brunetti, M.A, Lucini, E.I, Scorcione Turcato, M.C, Moreno, M.V, Frossasco, G.P, Colombatto, Martínez, M.J, Martínez Ferrer
• Format: Journal Article
• Language: English; Portuguese
• Published: Instituto Nacional de Tecnologia Agropecuaria (INTA) 01-04-2018
• Subjects: AGRICULTURE, DAIRY & ANIMAL SCIENCE; Methane; Fermentación ruminal; Natural additive; Rumen fermentation; Plant secondary compound; Metano; Aditivos naturales; Compuestos secundarios de plantas
• ISSN: 1669-2314

The aim of this study was to evaluate the effect of Argentinean essential oils (EO) on methane production during in vitro fermentation compared to EO with proven effects as rumen fermentation modifiers. A complete randomized block design was used and the treatments included EO from Aloysia gratissima (50, 100, 150 and 300 mg/L), Eucalyptus globulus (5, 50, 150 and 300 mg/L), Lippia turbinata (30, 60, 120 and 240 mg/L), Mentha x piperita var. vulgaris (50, 100, 200 and 400 mg/L), Origanum vulgare ssp. hirtum cv. Compacto (0.5, 5, 50 and 250 mg/L) and cv. Mendocino (50, 150, 250 and 350 mg/L), Rosmarinus officinalis (100, 300, 500 and 700 mg/L), Schinus molle (75, 150, 300 and 600 mg/L), Tagetes minuta (5, 50, 125 and 250 mg/L), and Thymus vulgaris (5, 50, 150 and 300 mg/L). Two controls were included: control (not containing EO) and monensin (1.87 mg/L). Variables measured were: digestibility of neutral detergent fiber, gas and methane production. Compared to the control, monensin reduced methane production by 44%, but with a 15% reduction in fiber digestibility. Except for E. globulus and L. turbinata, each variable measured was significantly reduced with the higher level of inclusion compared to the control, representing that overall microbial activity was affected. L. turbinata (60.8% limonene) reduced methane by half compared to control, and by 35 to 85% when compared to monensin, without affecting digestibility of the fiber. Some essential oils from native Argentinean plants exhibited great potential to reduce enteric methane production without affecting digestibility, of which L. turbinata was the most promising alternative.