基于广泛靶向代谢组学揭示鹅肥肝形成过程中代谢物动态变化规律

S835; 为探究鹅肥肝形成过程中代谢物组成及动态变化规律,利用广泛靶向代谢组学技术分析3个填饲阶段的鹅肝脏代谢谱.选取同批次体况相近的70日龄朗德鹅,分别在填饲前期(7d)、填饲中期(16d)、填饲后期(25 d)随机选取3只鹅屠宰,采集肝大叶肝尖组织样,用于广泛靶向代谢组学分析.结果表明:1)3个填饲阶段的鹅肝脏共检出氨基酸类、有机酸类、核苷酸类、脂类等19类,共1 153个代谢物.2)通过主成分分析发现,3个填饲阶段鹅肝脏代谢谱存在明显差异,填饲前期与中期、中期与后期分别鉴定出142、92个差异代谢物,这些代谢物以氨基酸及其衍生物、有机酸及其衍生物为主.3)通过代谢通路分析发现,鹅肥肝形...

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Published in:	食品科学 Vol. 45; no. 1; pp. 118 - 124
Main Authors:	马秋霞, 王宝维, 张名爱, 贾一铭, 孙丽骞, 张紫涵, 王思仪, 王悦, 伏莹莹, 孔敏, 凡文磊
Format:	Magazine Article
Language:	Chinese
Published:	青岛农业大学食品科学与工程学院,山东青岛 266109%青岛农业大学动物科技学院,山东青岛 266109 15-01-2024
Subjects:	differential metabolites widely targeted metabolomics 差异代谢物广泛靶向代谢组学 different overfeeding periods 不同填饲期 goose fatty liver 鹅肥肝
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Abstract	S835; 为探究鹅肥肝形成过程中代谢物组成及动态变化规律,利用广泛靶向代谢组学技术分析3个填饲阶段的鹅肝脏代谢谱.选取同批次体况相近的70日龄朗德鹅,分别在填饲前期(7d)、填饲中期(16d)、填饲后期(25 d)随机选取3只鹅屠宰,采集肝大叶肝尖组织样,用于广泛靶向代谢组学分析.结果表明:1)3个填饲阶段的鹅肝脏共检出氨基酸类、有机酸类、核苷酸类、脂类等19类,共1 153个代谢物.2)通过主成分分析发现,3个填饲阶段鹅肝脏代谢谱存在明显差异,填饲前期与中期、中期与后期分别鉴定出142、92个差异代谢物,这些代谢物以氨基酸及其衍生物、有机酸及其衍生物为主.3)通过代谢通路分析发现,鹅肥肝形成过程中发生显著变化的通路主要涉及脂肪酸生物合成、VB6代谢、亚油酸代谢、赖氨酸降解、精氨酸生物合成、花生四烯酸代谢、氨基酸的生物合成等.4)本研究发现,在鹅肥肝形成过程中差异代谢物大多涉及脂肪酸合成,转运相关的代谢物含量表现出持续增加的趋势.本研究揭示了填饲不同阶段鹅肝脏代谢图谱的变化规律,不仅丰富了家禽肝脏代谢的理论知识,还将为优质鹅肥肝的精准营养调控和高效生产提供理论依据.
AbstractList	S835; 为探究鹅肥肝形成过程中代谢物组成及动态变化规律,利用广泛靶向代谢组学技术分析3个填饲阶段的鹅肝脏代谢谱.选取同批次体况相近的70日龄朗德鹅,分别在填饲前期(7d)、填饲中期(16d)、填饲后期(25 d)随机选取3只鹅屠宰,采集肝大叶肝尖组织样,用于广泛靶向代谢组学分析.结果表明:1)3个填饲阶段的鹅肝脏共检出氨基酸类、有机酸类、核苷酸类、脂类等19类,共1 153个代谢物.2)通过主成分分析发现,3个填饲阶段鹅肝脏代谢谱存在明显差异,填饲前期与中期、中期与后期分别鉴定出142、92个差异代谢物,这些代谢物以氨基酸及其衍生物、有机酸及其衍生物为主.3)通过代谢通路分析发现,鹅肥肝形成过程中发生显著变化的通路主要涉及脂肪酸生物合成、VB6代谢、亚油酸代谢、赖氨酸降解、精氨酸生物合成、花生四烯酸代谢、氨基酸的生物合成等.4)本研究发现,在鹅肥肝形成过程中差异代谢物大多涉及脂肪酸合成,转运相关的代谢物含量表现出持续增加的趋势.本研究揭示了填饲不同阶段鹅肝脏代谢图谱的变化规律,不仅丰富了家禽肝脏代谢的理论知识,还将为优质鹅肥肝的精准营养调控和高效生产提供理论依据.
Abstract_FL	To understand the composition and dynamic changes of metabolites during the formation of goose fatty liver,the metabolite profiles of goose liver at three overfeeding stages were analyzed using widely targeted metabolomics.Three 70-day-old Landes geese with similar body conditions from the same batch were selected randomly for slaughter at the early(day 7),middle(day 16)and late(day 25)overfeeding stages,separately.The tip of the larger liver lobe was collected for widely targeted metabolomic analysis.The results showed that:(1)a total of 1 153 metabolites belonging to 19 classes including amino acids,organic acids,nucleotides and lipids were detected in the liver of geese at the three overfeeding stages;(2)principal component analysis(PCA)showed significant differences in the metabolic profiles of goose liver at the three stages,and identified 142 and 92 differential metabolites at the early versus middle stage,and the middle versus late stage,respectively,the major ones being amino acids and their derivatives,as well as organic acids and their derivatives;and(3)Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathway analysis indicated that the pathways involved in fatty acid biosynthesis,vitamin B6 metabolism,linoleic acid metabolism,lysine degradation,arginine biosynthesis,arachidonic acid metabolism and amino acid biosynthesis changed significantly during the formation of goose fatty liver.This study found that most of the differential metabolites were involved in fatty acid synthesis during goose fatty liver formation.Moreover,the contents of transport-related metabolites showed a continuous increasing trend.Findings in this study will not only enrich the theoretical knowledge of poultry liver metabolism,but also provide a theoretical basis for the precise nutritional regulation and efficient production of high-quality goose fatty liver.
Author	孔敏凡文磊王思仪王悦伏莹莹贾一铭孙丽骞张名爱马秋霞张紫涵王宝维
AuthorAffiliation	青岛农业大学食品科学与工程学院,山东青岛 266109%青岛农业大学动物科技学院,山东青岛 266109
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Author_FL	WANG Yue ZHANG Ming'ai WANG Siyi WANG Baowei ZHANG Zihan KONG Min FU Yingying FAN Wenlei SUN Liqian MA Qiuxia JIA Yiming
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DocumentTitle_FL	Widely Targeted Metabolomics Revealed the Dynamic Changes of Metabolites during the Formation of Goose Fatty Liver
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Title	基于广泛靶向代谢组学揭示鹅肥肝形成过程中代谢物动态变化规律
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