Solution structure of the orphan nuclear receptor Rev-erb beta response element by H, P NMR and molecular simulation

• Published in: Biochimie Vol. 82; no. 8; pp. 739 - 748
• Main Authors: Castagne, Claire, Terenzi, Hernan, Zakin, Mario M, Delepierre, Muriel
• Format: Journal Article
• Language: English
• Published: 01-08-2000
• ISSN: 0300-9084
• DOI: 10.1016/S0300-9084(00)01148-2

Rev-erb beta is an orphan receptor that binds as a homodimer or as a monomer to DNA. The solution structure of the non-palindromic 15 bp DNA duplex d(TAGAATGTAGGTCAG), the response element of Rev-erb beta for monomeric binding, was determined by 1H and 31P NMR, energy minimization with NMR-derived restraints for distances and NOE back-calculation methods. The refined final structures have the typical overall features of B-type DNA. However, titration of this 15 bp duplex with ReDBD, the DNA binding domain of Rev-erb beta , showed large shifts of imino protons and 31P signals, suggesting major conformational changes.