Is ribosomal cistron activity increased in human lymphoblastoid cell lines?

The genomic activity of nucleolar organizer regions (NORs) in eight human B-cell lymphoblastoid cell lines was studied following the routinely used Ag-NOR technique. The results demonstrate that (a) Ag-NORs are located in the short arms of D- and G-group chromosomes, (b) two out of eight cell lines...

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Bibliographic Details
Published in:	In vivo (Athens) Vol. 6; no. 2; p. 125
Main Authors:	Gupta, N J, Hopwood, V L, Pathak, S
Format:	Journal Article
Language:	English
Published:	Greece 01-03-1992
Subjects:	B-Lymphocytes - metabolism B-Lymphocytes - ultrastructure Cell Line, Transformed Cell Transformation, Neoplastic - metabolism Chromosome Aberrations Chromosomes, Human - ultrastructure DNA, Ribosomal - metabolism Gene Expression Humans Nucleolus Organizer Region - metabolism Nucleolus Organizer Region - ultrastructure RNA, Ribosomal - biosynthesis Silver Staining
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Summary:	The genomic activity of nucleolar organizer regions (NORs) in eight human B-cell lymphoblastoid cell lines was studied following the routinely used Ag-NOR technique. The results demonstrate that (a) Ag-NORs are located in the short arms of D- and G-group chromosomes, (b) two out of eight cell lines have 66.7% and 49.0% of metaphases, respectively, with 9 to 10 active Ag-NORs, and (c) as a whole, Ag-NOR activity is much higher in B-cell lines as compared with conventional 72-hr peripheral blood lymphocyte (T-cell) harvests.
ISSN:	0258-851X