A02 in southern Thai Muslims and central Thais

• Published in: Journal of the Medical Association of Thailand Vol. 80 Suppl 1; p. S25
• Main Authors: Chiewsilp, P, Mongkolsuk, T, Sujirachato, K
• Format: Journal Article
• Language: English
• Published: Thailand 01-09-1997
• Subjects: Alleles; Chi-Square Distribution; China - ethnology; Ethnic Groups - genetics; Gene Frequency; Haplotypes; HLA-A Antigens - classification; HLA-A Antigens - genetics; Humans; Malaysia - ethnology; Polymerase Chain Reaction - methods; Thailand; Thailand; China; Malaysia
• ISSN: 0125-2208

The HLA-A*02 subtyping in Thais was conducted and included in the 12th International Histocompatibility Workshop (12WS). A total of 81 randomized individuals previously serologically or DNA typed as A2 were studied for A2 subtypings. The subjects consisted of 32 Southern Thai-Muslims (STM) and 49 Central Thais (CT). The 12WS HLA-A*02 subtyping DNA typing kit was employed. The most common A*02 subtypes in STM were A*0203,*0201 and *0207 while they were A*0203, *0207 and *0201 in CT. A*0202, *0204, *0208, *0209, *0212, *0213, *0214, *0215, *0216 and *0217 were not found in both STM and CT. The 12WS data indicated that A*0201 was also the most frequent allele of A*2 among North-East Asians. A2 subtype study in 32 STM revealed that 2 in 8 of A*0201 showed the absence of bands at 813 bp and 705 bp with primer mix number 03A and 517A and weak reaction band with primer mix number 33A. In addition, 3 subjects with A*0201 variations have one nucleotide difference in exon 2 by sequence base typing (by MGJ. Tilanus) which will be reported separately.
More variations of A*02 were observed among STM compared to CT. The variations of reactions with the set of primer mix should be carefully observed and subjected to further analysis.