Optimization of supercritical CO sub(2) extraction of dried Helichrysum italicum flowers by response surface methodology: GC-MS profiles of the extracts and essential oil

• Published in: Separation science and technology Vol. 51; no. 18; pp. 2925 - 2931
• Main Authors: Jerkovic, Igor, Rajic, Marina, Marijanovic, Zvonimir, Bilic, Mate, Jokic, Stela
• Format: Journal Article
• Language: English
• Published: 01-09-2016
• Subjects: Acetates; Carbon dioxide; Derivatives; Extraction; Flowers; Helichrysum; Optimization; Process parameters; Response surface methodology
• ISSN: 0149-6395; 1520-5754
• DOI: 10.1080/01496395.2016.1237967

Supercritical (SC) CO sub(2) extraction of dried Immortelle flowers was performed at different process parameters. The optimal extraction conditions related to the yield were determined by response surface methodology (yield 4.09% at 20 MPa and 52 degree C). The extracts were analyzed by GC-MS and tremetone derivatives dominated: bitalin A, 12-acetoxytremetone, gnaphaliol, 1-[2-(2-methyl-2,3-dihydroxypropyl)-2,3-dihydro-1-benzofuran-5-yl] e thanone, isobutyl bitalin A, and 1-[2-(acetylprop-1-en-2-yl)-3-hydroxy-2,3-dihydro-1-benzofuran-5-y l ]ethanone. Striking differences were found among the essential oil and SC-CO sub(2) extracts composition. The major oil compounds were gamma -curcumene, alpha -pinene, beta -selinene, alpha -selinene, and limonene. Mono- and sesquiterpene were found among minor constituents of the extracts. Neryl acetate was present in the extracts and essential oil.