2a2-O-{[2,2-dimethyl-2-(2-nitrophenyl) acetyl] oxy} methyl protecting group for RNA synthesis

• Published in: Tetrahedron letters Vol. 54; no. 32; pp. 4281 - 4284
• Main Authors: Chen, Ke, Wang, Wei, Qu, Dezhong, Zhao, Haoting, Xiong, Wei, Luo, Caijie, Yin, Menghui, Zhang, Biliang
• Format: Journal Article
• Language: English
• Published: 01-08-2013
• Subjects: Compatibility; Joining; Oligonucleotides; Reduction; Ribonucleic acids; Solid phase synthesis; Synthesis (chemistry); Tetrahedrons
• ISSN: 0040-4039
• DOI: 10.1016/j.tetlet.2013.06.001

A new 2a2-OH protecting group, {[2,2-dimethyl-2-(2-nitrophenyl)acetyl]oxy} methyl (DAM), has been successfully applied to RNA oligonucleotides synthesis with high coupling efficiency. The 2a2-O-DAM method is fully compatible with reagents and conditions employed in automated solid phase synthesis of RNA. Moreover, it can be easily removed via reduction in near-neutral solution.