Molecular research methods in the detection of germinal mutations in hereditary colorectal cancer

Molecular research methods in the detection of germinal mutations in hereditary colorectal cancer

Colorectal cancer (CRC) is one of the main causes of death in South American countries. The hereditary forms of CRC are, familial adenomatous (FAP) and hereditary nonpolyposis colorectal cancer (HNPCC) or Lynch Syndrome (LS), which is the most common form. The detection of mutations in the DNA repai...

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Bibliographic Details
Published in:Revista de gastroenterología del Perú Vol. 29; no. 3; pp. 247 - 253
Main Authors: Dominguez, M V, Bastos, E P, Silva, S D, Rossi, B M
Format: Journal Article
Language:Spanish
Published: Peru 01-07-2009
Subjects:
Colorectal Neoplasms - genetics
DNA Mutational Analysis - methods
Humans
Mutation
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Summary:Colorectal cancer (CRC) is one of the main causes of death in South American countries. The hereditary forms of CRC are, familial adenomatous (FAP) and hereditary nonpolyposis colorectal cancer (HNPCC) or Lynch Syndrome (LS), which is the most common form. The detection of mutations in the DNA repair genes (MMR) and in the APC genes enables the development of prevention strategies. Some of these methods for molecular diagnosis are applied in research and the detection of mutations of these genes, such as the partial thromboplastin time test (PTT), the single strand conformational polymorphism test (SSCP), the Denaturing High Performance Liquid Chromatography test (DHPLC) and the Polymerase Chain Reaction (PCR) in real time (qPCR).
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ISSN:1022-5129