Interleukin-10 inhibits expression of interleukin-6 and -8 mRNA in human dental pulp cell cultures via nuclear factor-kappaB deactivation

• Published in: Journal of endodontics Vol. 28; no. 3; p. 177
• Main Authors: Tokuda, Masayuki, Nagaoka, Shigetaka, Torii, Mitsuo
• Format: Journal Article
• Language: English
• Published: United States 01-03-2002
• Subjects: Adolescent; Blotting, Northern; Cells, Cultured; Dental Pulp - cytology; Dental Pulp - metabolism; Fibroblasts - metabolism; Gene Expression Regulation - drug effects; Humans; Interleukin-10 - blood; Interleukin-10 - pharmacology; Interleukin-6 - antagonists & inhibitors; Interleukin-6 - biosynthesis; Interleukin-8 - antagonists & inhibitors; Interleukin-8 - biosynthesis; Lipopolysaccharides - pharmacology; Male; NF-kappa B - antagonists & inhibitors; NF-kappa B - metabolism; Prevotella intermedia - chemistry; Protein Binding - drug effects; Recombinant Proteins - pharmacology; RNA, Messenger - analysis
• ISSN: 0099-2399

The effects of interleukin (IL)-10 on the expression of IL-6 and IL-8 mRNA in human dental pulp cell cultures were investigated by using the Northern blot analysis. On stimulation with Prevotella intermedia lipopolysaccharide (PiLPS), IL-10 was produced in peripheral blood but was not detected in human dental pulp cell culture supernatants. IL-10 inhibited IL-8 mRNA expression, which is normally stimulated by PiLPS, IL-1alpha, and tumor necrosis factor-alpha and inhibited IL-6 mRNA expression, which is normally stimulated by IL-1alpha. In addition, IL-10 inhibited the activation of nuclear factor-kappaB, which is normally induced by PiLPS. We conclude that IL-10 inhibits expression of IL-6 and IL-8 mRNA in dental pulp cell cultures by inhibiting the activation of nuclear factor-kappaB.