Detection of mycobacteria tuberculosis in patients with urogenital tuberculosis by PCR method

• Published in: Georgian medical news no. 119; p. 14
• Main Authors: Dochviri, T Z, Katsitadze, V A, Khosiashvili, G Z, Chigogidze, T G
• Format: Journal Article
• Language: Russian
• Published: Georgia (Republic) 01-02-2005
• Subjects: Adult; Aged; Algorithms; Bacteriological Techniques; DNA, Bacterial - isolation & purification; DNA, Bacterial - urine; Female; Humans; Male; Middle Aged; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Polymerase Chain Reaction; Tuberculosis, Urogenital - diagnosis; Tuberculosis, Urogenital - microbiology; Tuberculosis, Urogenital - urine; Urine - microbiology
• ISSN: 1512-0112

The study was carried out in hospital patients as well as in outpatients at the National Centre of Tuberculosis and Lung Diseases of Georgia (2002-2004). The group consisting of 32 patients with tuberculosis of urogenital system has been studied (newly detected forms). Except clinical laboratory, culture and X-ray contrast methods, two additional methods were used in testing of this group of patients. The examination of their urine, at the same time, was carried out by the Polymerase Chain Reaction method in order to detect Kochi bacillus and by three-time bacterioscopy of urine for acid resistant bacteria. Mycobacterium tuberculosis in urine has been detected in 26 (81,25%) patients by PCR method, and by urine bacterioscopy--acid fast bacilli (AFB+) in 18 (56,25%) patients. The histo-morphological investigation of specimens obtained by surgery confirmed the TB diagnosis in all patients. This study on patients suspected of Tuberculosis of genital-urinary system gives us an opportunity to update the diagnostic algorithm by including the modern molecular methods. This algorithm will help in timely detection of Tuberculosis, in selection of adequate therapy and in prevention of the further progression of the disease.