Radiation mapping of the short arm of swine chromosome 2

In recent years, maps of mammalian genomes have been acquiring increasingly higher resolution. Integration of maps of different types has become possible. As a tool in integrating maps of mammalian genomes of different types, high-resolution mapping with radiation-induced hybrids (RH) is used. Here,...

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Bibliographic Details
Published in:	Genetika Vol. 37; no. 2; p. 230
Main Authors:	Ivanova, E V, Koroleva, I V, Kuznetsov, S B, Aksenovich, T I, Svishcheva, G R, Mal'chenko, S N, Bendixen, C, Zhdanova, N S
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-02-2001
Subjects:	Animals Base Sequence Chromosome Mapping - veterinary DNA Primers Hybrid Cells - radiation effects Mink - genetics Polymerase Chain Reaction Swine - genetics
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Summary:	In recent years, maps of mammalian genomes have been acquiring increasingly higher resolution. Integration of maps of different types has become possible. As a tool in integrating maps of mammalian genomes of different types, high-resolution mapping with radiation-induced hybrids (RH) is used. Here, we present an RH6000 map of the short arm of porcine chromosome 2. The map contains 15 microsatellites and five genes (for parathyroid hormone, lactate dehydrogenase A, myogenic factor, follicle-stimulating hormone beta, and calpain I). The RH panel was obtained on the basis of a hybrid cell line bearing the single porcine chromosome 2 against the background of mink chromosomes. The mean frequency of preserving markers examined in the panel was 18.3%. Integration of four genes in the panel and a comparison of gene order in homologous regions of human and porcine chromosomes are presented.
ISSN:	0016-6758