Effect of cations and DNP on ATPase activity in different tissue fractions of birds' brains

• Published in: Voprosy biokhimii mozga Vol. 9; p. 219
• Main Authors: Simonian, A A, Stepanian, R A, Batikian, G G
• Format: Journal Article
• Language: Russian
• Published: Armenia (Republic) 1974
• Subjects: Adenosine Triphosphatases - metabolism; Animals; Brain - enzymology; Brain - ultrastructure; Calcium - pharmacology; Cations - pharmacology; Chickens - metabolism; Dinitrophenols - pharmacology; Freezing; Magnesium - pharmacology; Mitochondria - enzymology; Potassium - pharmacology; Sodium - pharmacology
• ISSN: 0507-2972

ATP-ase activity and the effect of cations (Na+, K+, Mg2+, Ca2+) and DNP on its activity have been studied in avian brain homogenates and mitochondrial and supernatant fractions (microsomes + hyaloplasma). Highest levels of Na+ + K+ -stimulated ATP-ase activity has been shown in avian brain crude mitochondrial fraction, next in supernatant and lowest in homogenates. Following three-fold freezing and thawing, enzyme activity in homogenates and mitochondrial fractions increases considerably and decreases in the supernatant. Highest Mg2+ -stimulated ATP-ase activity is found in avian brain homogenates, next in mitochondrial fractions and supernatants. Mg2+ -stimulated ATP-ase activity is not changed considerably following three-fold freezing and thawing of brain preparations. Highest Ca2+ -stimulated ATP-ase activity has been observed in the supernatant fraction and its activity is considerably reduced following freezing and thawing. Avian brain mitochondrial fractions have high DNP-stimulated ATP-ase activity. Following freezing and thawing of brain preparations DNP-stimulated ATP-ase activity is not changed considerably. The results obtained indicate that in avian brain homogenates and other preparations, cation and DNP-stimulated ATP-ase have different activities. Three-fold freezing and thawing of brain tissue preparations affect these activities differentially.