Monocyte chemotaxis: critical evaluation of 2 methods of quantification

• Published in: Allergologia et immunopathologia Vol. 12; no. 2; p. 123
• Main Authors: Jiménez López, A, Olmos Martínez, J M, García Palomo, J D, Sánchez Hernández, J J, De Dios Martín, B, Pedraz García, M J
• Format: Journal Article
• Language: Spanish
• Published: Spain 01-03-1984
• Subjects: Adult; Chemokines, C; Chemotaxis, Leukocyte; Cytological Techniques; Endotoxins - pharmacology; Female; Humans; Lymphokines; Male; Middle Aged; Monocytes - physiology; Phagocytosis; Sialoglycoproteins; Technetium; Zymosan - pharmacology
• ISSN: 0301-0546

The mononuclear phagocytic system (Mo) possesses a series of well defined functions regarding both its circulatory components--monocytes--and histiocytic macrophages. These functions--antimicrobial defense, elimination of cellular particles or detritus, immunological interaction with lymphocytes cells, antitumoral defense and control of granulopoiesis--require chemotaxis and phagocytosis or preceding steps. Although knowledge of these functional aspects is continually becoming better--always assured by advances obtained in the study of the function and pathology of polymorphonuclear cells--, an authentic pathology of monocyte chemotaxis as an independent clinical entity doesn't exist. This is attributed to of these cells. The techniques currently in use differ not only in their methodology but also in their bases and thus the results obtained by various authors are not generally comparable. All this led us to do a comparative study, in control subjects, between two techniques which explore the chemotactic capacity of monocytes from peripheral blood. The chemotactic capacity of monocytes from peripheral blood in control subjects, measured by two distinct quantification methods, were studied: Radioanalytic Method (monocytes tagged with 99 mTc) and a morphological method (counting the number of monocytes per field in immersion). The results obtained from both techniques were very similar, the existence of significant differences in cell behavior with exposure to the different chemotactic substances used could not be demonstrated. These results are commented on.