Monoclonal antibody-based immunoenzyme test system for determining the total immunoglobulin E level of children and adolescents

Enzyme immunoassay for determination of total immunoglobulin E in children and adolescents based on combination of two monoclonal antibodies with high affinity and specificity to different epitopes of IgE molecule has been developed. Monoclonal antibodies IgE/11-4 are used for sensitization of a sol...

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Bibliographic Details
Published in:	Biulleten' eksperimental'noi biologii i meditsiny Vol. 107; no. 6; p. 722
Main Authors:	Serdiuk, O A, Tsytsikov, E N, Vasilov, R G
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-06-1989
Subjects:	Adolescent Antibodies, Monoclonal - isolation & purification Antibody Specificity Anticoagulants - pharmacology Child Evaluation Studies as Topic Humans Immunoenzyme Techniques - instrumentation Immunoglobulin E - analysis
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Summary:	Enzyme immunoassay for determination of total immunoglobulin E in children and adolescents based on combination of two monoclonal antibodies with high affinity and specificity to different epitopes of IgE molecule has been developed. Monoclonal antibodies IgE/11-4 are used for sensitization of a solid phase (96-well plate), mcAB IgE/11-1 conjugated with horseradish peroxidase are used as a developing reagent. The following analytical characteristics of a test-system are established: range of assay--1-100 IU/ml sensitivity--1 IU/ml; time of analysis--1.5 hour, sample volume in duplicates--30 microliters. Immunoassay is simple, reliable and can be used in the field of pediatrics and allergology.
ISSN:	0365-9615