Study of the participation of the alpha-chain of the LFA-1 antigen in the phenomenon of lymphocyte adherence inhibition using ICO-11 monoclonal antibodies in vitro

• Published in: Biulleten' eksperimental'noi biologii i meditsiny Vol. 104; no. 12; p. 711
• Main Authors: Kharkevich, D D, Baryshnikov, A Iu, Savel'eva, E V, Polevaia, E B, Kadagidze, Z G
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-12-1987
• Subjects: Antibodies, Monoclonal - immunology; Antigens, Surface - immunology; Breast Neoplasms - immunology; Female; Humans; Immunologic Techniques; In Vitro Techniques; Leukocyte Adherence Inhibition Test; Lymphocyte Function-Associated Antigen-1
• ISSN: 0365-9615

The influence of monoclonal antibodies (Mabs) ICO-11 on the ability of lymphocytes from breast cancer patients to react in lymphocyte adherence inhibition (LAI) test has been studied in vitro. It has been demonstrated that Mabs ICO-11 in the dilutions 1/4 and higher blocked the reaction to tumor extracts in LAI-test, without affecting the reaction to the extracts of normal tissues and a spontaneous adhesion of lymphocytes of healthy donors as well. The addition of the control supernatants of myeloma cells X63.Ag8. 653 to the test-system in the same dilutions caused no influence upon LAI-reaction and spontaneous adhesion of lymphocytes from healthy donors. A possible participation of alpha-chains of function-associated antigens in the binding of tumor-associated antigens to T-cells in the inductive phase of the reaction of lymphocyte adherence inhibition in vitro has been suggested.