Methods of isolating and selecting recombinant vaccinia viruses expressing heterogenetic viral antigens

The paper describes a method using plasmid construction pSC11 for generation of recombinant vaccinia viruses supporting coexpression of heterologous genes and beta-galactosidase. The Ca2+-phosphate method of cell transfection by recombinant DNAs generated on the basis of pSC11, and selection of reco...

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Bibliographic Details
Published in:	Voprosy virusologiĭ Vol. 33; no. 4; p. 428
Main Authors:	Gibadulin, R A, Iuferov, V P, Lideman, L F, Parasiuk, N A, Ionova, K S
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-07-1988
Subjects:	Antigens, Heterophile - genetics Antigens, Viral - genetics DNA, Viral - genetics Gene Expression Regulation Genes, Viral Genetic Techniques Hemagglutinins, Viral - genetics Plasmids Recombination, Genetic Selection, Genetic Transfection Vaccinia virus - genetics Vaccinia virus - immunology Vaccinia virus - isolation & purification
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Summary:	The paper describes a method using plasmid construction pSC11 for generation of recombinant vaccinia viruses supporting coexpression of heterologous genes and beta-galactosidase. The Ca2+-phosphate method of cell transfection by recombinant DNAs generated on the basis of pSC11, and selection of recombinant viruses from blue plaques of virus-infected cells in the presence of X-gala are reported at length.
ISSN:	0507-4088