Demonstration of maturation promoting factor activity in Saccharomyces cerevisiae

Microinjection of a crude yeast extract into fully grown immature Xenopus laevis oocytes induces the reinitiation of meiotic maturation as does Xenopus laevis oocyte MPF (Maturation Promoting Factor) by itself. Using several strains of yeast exhibiting temperature sensitive cell cycle block (cdc), i...

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Bibliographic Details
Published in:	Comptes rendus des séances de l'Académie des sciences. Série 3, Sciences de la vie Vol. 295; no. 13; p. 787
Main Authors:	Weintraub, H, Buscaglia, M, Ferrez, M, Weiller, S, Boulet, A, Fabre, F, Baulieu, E E
Format:	Journal Article
Language:	French
Published:	France 20-12-1982
Subjects:	Animals Cell Cycle - drug effects Female Growth Substances - analysis Growth Substances - pharmacology In Vitro Techniques Maturation-Promoting Factor Mutation Oocytes - drug effects Saccharomyces cerevisiae - analysis Xenopus laevis
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Summary:	Microinjection of a crude yeast extract into fully grown immature Xenopus laevis oocytes induces the reinitiation of meiotic maturation as does Xenopus laevis oocyte MPF (Maturation Promoting Factor) by itself. Using several strains of yeast exhibiting temperature sensitive cell cycle block (cdc), it was observed that yeast extract exhibits MPF activity only when obtained during the time interval from the G2 period to the metaphase. This observation stresses eucaryotic ubiquity of MPF.
ISSN:	0249-6313