Cloning fragments of the RNA polymerase gene of an attenuated variant of the foot-and-mouth disease virus A22

The cDNA fragments complementary to RNA-polymerase gene and 3'-untranslated genome region of attenuated foot-and-mouth disease virus strain A(22)645 have been synthesized and cloned into a plasmid vector pUC19 in E. coli JM109. The cloned cDNA fragments were characterized as to their size, orie...

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Bibliographic Details
Published in:	Molekulârnaâ genetika, mikrobiologiâ i virusologiâ no. 2; p. 33
Main Authors:	Lomakina, N F, Falina, G M, Shcherbakov, A V, Drygin, V V, Driagalin, N N, Bykova, L A, Rybakov, S S
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-04-1995
Subjects:	Aphthovirus - enzymology Aphthovirus - genetics Aphthovirus - immunology Cloning, Molecular DNA, Complementary DNA-Directed RNA Polymerases - chemistry DNA-Directed RNA Polymerases - genetics Escherichia coli - genetics Peptide Fragments - genetics Plasmids Vaccines, Attenuated - genetics Viral Vaccines - genetics
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Summary:	The cDNA fragments complementary to RNA-polymerase gene and 3'-untranslated genome region of attenuated foot-and-mouth disease virus strain A(22)645 have been synthesized and cloned into a plasmid vector pUC19 in E. coli JM109. The cloned cDNA fragments were characterized as to their size, orientation towards the plasmid, and localization in the virus genome. Restriction maps for complete gene and two cDNA clones were constructed.
ISSN:	0208-0613