Analysis of miniature potentials in reinnervated rat muscle

• Published in: Bollettino della Societa italiana di biologia sperimentale Vol. 67; no. 7; p. 667
• Main Authors: Fulgenzi, G, Cuppini, R, Bruno, C
• Format: Journal Article
• Language: Italian
• Published: Italy 01-07-1991
• Subjects: Acetylcholine - secretion; Action Potentials; Animals; Male; Motor Endplate - physiology; Nerve Crush; Nerve Regeneration - physiology; Rats; Rats, Inbred Strains; Sciatic Nerve - physiology
• ISSN: 0037-8771

Miniature endplate potentials (MEPPs) are regarded as the expression of release of a single quantum of acetylcholine by motor nerve endings in the muscle. Mepp frequency is dependent on the presynaptic mechanism, but MEPP amplitudes and time courses are the result of the characteristics of pre- and postsynaptic structures and of the interaction between them. After post-traumatic reinnervation of skeletal muscles, MEPP frequency increases, reaching slowly normal values. Two groups of male, Sprague Dawley rats were used: in the first group left sciatic nerve was crushed and nerve fibres were allowed to regenerate, whereas the others were regarded as controls. MEPPs were intracellularly recorded in end plates of normal and reinnervated left extensor digitorum longus muscle. MEPPs were sampled and recorded on a personal computer, and, subsequently, amplitude, rise time and half decay time were computed. At early stage after reinnervation, MEPPs showed rise times and decay times longer than normal. Afterwards, we did not find differences between mepp time courses by normal and reinnervated end plates. The possible relationships between the results and changes in acetylcholine receptor number and type, and in acetylcholinesterase activity occurring during denervation and reinnervation are discussed.