Group determination of 17-oxosteroids on thin-layer chromotography

17-oxosteroids from human urine are measured by thin layer chromatography (Merck Kieselgel F-254 plates) with fluorometric detection at 310 nm (densitometer SHIMADZU CS-9000): 11-OH-etiocholanolone, 11-OH-androsterone + oxoetiocholanolone, oxoandrosterone, dehydroepiandrosterone and androsterone. Fl...

Full description

Saved in:

Bibliographic Details
Published in:	Sbornik lekarsky Vol. 93; no. 7-8; p. 232
Main Authors:	Tomsová, Z, Jůzová, Z
Format:	Journal Article
Language:	Czech
Published:	Czech Republic 01-08-1991
Subjects:	17-Ketosteroids - urine Chromatography, Thin Layer Humans
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	17-oxosteroids from human urine are measured by thin layer chromatography (Merck Kieselgel F-254 plates) with fluorometric detection at 310 nm (densitometer SHIMADZU CS-9000): 11-OH-etiocholanolone, 11-OH-androsterone + oxoetiocholanolone, oxoandrosterone, dehydroepiandrosterone and androsterone. Fluorescence of hydrazone derivatives after the reaction of steroids with dansylhydrazine represents the principle of the method. The recoveries of steroids added to human urine samples are 97-101.3% and the coefficients of variation are 0.78-11.77.
ISSN:	0036-5327